Test

The acid phosphatase test is widely used to identify stains of seminal origin. (1,2,3,4) Tests for acid phosphatase which is use a phenolic ester as a substrate are non-specific; experimental interferences were studied and a simple method of detection of interferences is reported.

The test methods consist of reacting the suspected stain or an extract of it with a solution of suitably buffered phenolic phosphate ester is hydrolyzed to phosphate ion and phenol. the phenol formed, if any, is then reacted with a third substance to form a colored product. The presence of this colored product indicates a positive test for the phosphatase. If the quantity of colored product is sufficient within the time and concentration limits stated in the test, a conclusion that the stain is of seminal origin is justified. The difficulty with this process is that extraneous phenols will react with the chromogenic color forming substance to produce colored substances which may interfere or produce a false positive reaction. With some very reactivee chromogenic substances, such as the diazoniom ions, other classes of chemicals, such as aromatic amines and their salts, imidazoles, pyrroles, indoles, ammonium ion, histidine, hydrazine, hydroxylamine, phenylalanine. tryptophane, tyrosine, arginine, also react to form colored products under suitable conditions.

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